The Mechanisms of A New Plant Lectin FRIL from Dolichos Lablab That Preserves Quiescent Hematopoietic Stem Cells in Suspension Culture

新的豆类凝集素FRIL及其体外维持造血干/祖细胞特性的作用机制

We have also found another receptor of FRIL by affinity purify and western blot with antibody to FRIL . It may be a new mediate to FRIL \'s function .

利用亲和纯化和自制受体的研究还发现FRIL可能存在Flt3之外的受体来介导它的功能。

But the underlying mechanism for maintaining quiescence of HSCs is largely unknown at present , so in this study , we focused on the mechanism of how FRIL maintains quiescence of HSCs through regulation of cell cycle .

但是其维持造血干祖细胞静息的机制目前还不清楚，因此本文从细胞周期调控的角度对这一问题进行了探讨。

To investigate the underlying mechanism of how FRIL maintain HSCs in vitro , we detected the expression of various cell cycle regulators , especially those involved in G0 / G1 phase regulation on mRNA and protein level .

为了探讨FRIL体外维持造血干／祖细胞的可能机制，本实验对参与细胞周期调控，尤其是参与G0／G1期细胞周期调控的多种因子进行了mRNA水平及蛋白水平的研究。

Concanavalin A ( Con A ), is a kind of lectin separated from sword bean . A newly cloned lectin , FRIL ( Flt3 receptor-interacting lectin ), was identified to be capable of preserving primitive progenitors in vitro without loss of repopulating capacity .

最近发现的一种植物凝集素FRIL（Flt3receptor-interactinglectin），能够于离体条件下长期维持造血干细胞的自我更新能力。