Regene - rated calli , 2 & 4 mm in diameter , were transferred in succession on differentiation medium Z_3 and Z_4 for organogenesis .

待再生愈伤组织长到直径2&4mm大小时，把它们先后转经第一及第二（即Z_3及Z_4）分化培养基上诱导器官分化。